Journal: Oncology Research

Article Title: Promoter Hypermethylation-Driven NPHS2 Silencing Promotes Immune Escape and Sunitinib Resistance in Clear Cell Renal Cell Carcinoma

doi: 10.32604/or.2026.080228

Figure Lengend Snippet: The effect of NPHS2 on ccRCC cell activity. ( A ) IC 50 for sunitinib in resistant and normal cells. ( B ) Growth curves of 786-O, A498, 786-O/R, and A498/R ( n = 3). ( C ) Representative fluorescence images showing GFP-positive cells after lentiviral infection in 786-O, A498, 786-O/R, and A498/R cells ( n = 3). ( D ) Validation of the effect of intervention on NPHS2 expression ( n = 3). ( E ) EdU detection of the effect of NPHS2 on the proliferative capacity of 786-O, A498, 786-O/R, and A498/R ( n = 3). ( F ) Cell cloning assay to detect the effect of NPHS2 on the cloning ability of 786-O, A498, 786-O/R, and A498/R ( n = 3). ( G ) Transwell assay to detect the effect of NPHS2 on 786-O, A498, 786-O/R, and A498/R invasion ability ( n = 3). ( H ) Cell scratch assay to detect the effect of NPHS2 on the migration ability of 786-O, A498, 786-O/R, and A498/R ( n = 3). ( I ) Effect of flow cytometry on NPHS2 in 786-O, A498, 786-O/R, and A498/R cell cycle. ( J ) Expression of apoptosis-related proteins ( n = 3). vs. NPHS2-nc group, * p < 0.05; vs. NPHS2-nc-R group, # p < 0.05.

Article Snippet: Sunitinib-resistant sublines were established by stepwise exposure to sunitinib at 2.5, 7.5, 12.5, and 20.0 μmol/L (SU11248, TargetMol Chemicals Inc., Boston, MA, USA).

Techniques: Activity Assay, Fluorescence, Infection, Biomarker Discovery, Expressing, Cloning, Transwell Assay, Scratch Assay, Migration, Flow Cytometry

Journal: NPJ Biofilms and Microbiomes

Article Title: Endogenous Coriobacteriaceae enriched by a high-fat diet promotes colorectal tumorigenesis through the CPT1A-ERK axis

doi: 10.1038/s41522-023-00472-7

Figure Lengend Snippet: A Schematic diagram of the experimental procedure for constructing C57BL/6J AOM mouse models and representative colon tumour images. B Cori .ST1911 abundance at 0, 1, 2, and 4 weeks determined using qPCR and normalised to universal bacterial primers by targeting 16S rRNA genes. C Tumour number and volume in C57BL/6J AOM mice ( n = 5) gavaged with Cori .ST1911, E. coli , or vehicle. Data are represented as mean ± SEM, analysed using one-way ANOVA. n.s., no significance. D Representative images of HE and nuclear Ki67 staining of the Cori .ST1911 group and GAM broth control group mice. Scale bars, 200 µm. Percentage of nuclear Ki67 + cells ( n = 5). Data are represented as mean ± SEM, analysed using two-tailed Student’s t-test. E Schematic diagram of the experimental procedure for constructing model Apc Min/+ mice and representative colon tumour images. F Tumour number and volume in Apc Min/+ mice ( n = 5) gavaged with Cori .ST1911, Cori .ST1911 culture supernatant, or vehicle. Data are represented as mean ± SEM, analysed using one-way ANOVA. n.s., no significance. G Schematic diagram of the experimental procedure for constructing a subcutaneous transplanted tumour model in BALB/c mice and representative colon tumour images. Mice inoculated with CT26 cells were randomly assigned to Cori .ST1911 and GAM broth control groups when the tumour reached 3–4 mm. Tumour tissue was harvested 5 weeks later. H Tumour weight and volume in BALB/c mice gavaged with Cori .ST1911 or vehicle. Data are represented as mean ± SEM, analysed using two-tailed Student’s t-test. SUP, supernatant.

Article Snippet: CT26 cells treated with phosphatase inhibitor (U0126-EtOH, HY-12013; SP600125, HY-12041; SB202190, HY-10295, TWS119, HY-10590; AR-A014418, HY-10512, MCE, USA)before Cori.ST1911 treatment and Cell OD was measured using the CCK8 method.

Techniques: Staining, Control, Two Tailed Test

Journal: NPJ Biofilms and Microbiomes

Article Title: Endogenous Coriobacteriaceae enriched by a high-fat diet promotes colorectal tumorigenesis through the CPT1A-ERK axis

doi: 10.1038/s41522-023-00472-7

Figure Lengend Snippet: A Percentage cell proliferation of CT26 cells treated with MEK inhibitor U0126-EtOH (25 µM), JNK inhibitor SP600125 (25 µM), P38/MAPK inhibitor SB20.2190 (20 µM), GSK3β inhibitor tws119 (20 µM), or AR-A014418 (20 µM), and vehicle or Cori .ST1911. Cell OD was measured using the CCK8 method and normalised to cell proliferation of the control. Inhibitor treatment started 4 h before Cori .ST1911 treatment. Data are represented as mean ± SEM ( n = 3 independent tests), analysed by one-way analysis of variance (ANOVA) with Bonferroni’s post-hoc test. n.s., no significance. B Representative western blots showing the effect of increasing Cori .ST1911 MOI (100-300) on CPT1A and p-ERK. C Representative western blots showing the effect of Cori .ST1911 on CPT1A and MAPK-related genes after treatment with SiCPT1A (48 h) or etomoxir (100 µM). Proteins were quantified densitometrically using ImageJ software and analysed using two-tailed Student’s t-test ( B , C ). D Schematic diagram of the experimental procedure of HFD and HFD-LC (L-carnitine, 100 mg/kg/day) treatment of C57BL/6J AOM mice, and representative colon tumour images. E Tumour number, tumour volume, and serum palmitoyl L-carnitine concentration in HFD- and HFD-LC-fed C57BL/6J AOM mice ( n = 6). Data are represented as mean ± SEM, analysed using two-tailed Student’s t-test. F Schematic diagram of the experimental procedure of HFD and HFD-metronidazole treatment of C57BL/6J AOM mice, and representative colon tumour images. G Tumour number, tumour volume, and serum palmitoyl L-carnitine concentration in HFD- and HFD-metronidazole-fed C57BL/6J AOM mice ( n = 6). Data are represented as mean ± SEM, analysed using two-tailed Student’s t-test. H Schematic diagram of the experimental procedure of Cori .ST1911 and Cori .ST1911-etomoxir treatment of subcutaneous transplanted tumour BALB/c mice, and representative colon tumour images. BALB/c mice were randomly divided into groups after subcutaneous injection of CT26 cells, followed by intragastric administration of Cori .ST1911 (1 × 10 8 CFU once every 2 days) and intraperitoneal injection of etomoxir (15 mg/kg, 3 times/week). I Representative western blots showing MAPK-related protein expression in HFD-, HFD-LC-, and HFD-metronidazole-fed mice. Proteins were quantified densitometrically using ImageJ software and analysed using two-tailed Student’s t-test. MOI, multiplicity of infection; LC, L-carnitine; ERK, extracellular regulated protein kinases; MEK, Mitogen-activated protein kinase; MAPK, mitogen-activated protein kinase.

Article Snippet: CT26 cells treated with phosphatase inhibitor (U0126-EtOH, HY-12013; SP600125, HY-12041; SB202190, HY-10295, TWS119, HY-10590; AR-A014418, HY-10512, MCE, USA)before Cori.ST1911 treatment and Cell OD was measured using the CCK8 method.

Techniques: Control, Western Blot, Software, Two Tailed Test, Concentration Assay, Injection, Expressing, Infection